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. /Marciana/Marciana/lo_que_es_la_mancha_y_el_mar_de_la_mancha.pdf.Fluorescence-detected coherent anti-Stokes Raman scattering microscopy of rat brain. Fluorescence-detected coherent anti-Stokes Raman scattering microscopy (FD-CARS) is a high-resolution fluorescence microscopy technique with the ability to simultaneously detect two vibrational modes, such as 1H NMR and CARS. Herein, CARS microscopy is used to image rat brain samples. Brain tissue samples were used as controls and rats (n = 4) were given a dose of 10 mL/kg/day of the nerve agent agent Sarin via intraperitoneal injection (i.p.) to produce a rat model of nerve agent exposure. We studied three different regions of the brain: the cerebral cortex, the hippocampal area, and the cerebellum. Using FD-CARS, we observed spatial distribution of CARS emissions in tissue and were able to discriminate different concentrations of biotinylated dextran amine in the cortical and cerebellar regions. Furthermore, we observed differences in the distribution of CARS emissions within the tissues based on region, and we observed changes in the spatial distribution of CARS emissions in the striatum and cerebellum following i.p. administration of Sarin. FILED NOT FOR PUBLICATION SEP 30 2011 MOLLY C

 

 

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